Journal: The Journal of comparative neurology

Article Title: Characterisation of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations

doi: 10.1002/cne.23648

Figure Lengend Snippet: Primary Antibodies Used for Immunohistochemistry

Article Snippet: Markers of non-neuronal cells were also used: S100 and glial fibrillary acidic protein (GFAP), glial cells; smooth muscle actin (SMA), smooth muscle; vimentin (VIM), expressed by interstitial cells. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Description of immunogen Supplier/catalogue number Dilution Calcitonin gene-related peptide (CGRP) GG-conjugated synthetic peptide corresponding to amino acids 23–37 of rat Tyr-CGRP Biogenesis (now AbD Serotec), goat polyclonal, Cat# 1720-9007, RRID:AB_2290729 1:2000 Calcitonin gene-related peptide (CGRP) KLH-conjugated rat synthetic peptide Millipore, rabbit polyclonal Cat# AB5920, RRID:AB_2068655 1:5000 Glial fibrillary acidic protein (GFAP) GFAP isolated from cow spinal cord Dako Cat# Z0334, rabbit polyclonal, RRID:AB_10013382 1:1000 Glial cell line-derived neurotrophic factor family receptor α3 (GFRα3) Recombinant protein corresponding to amino acid sequence Glu34-Arg379 of mouse GFRα3 (Accession # {"type":"entrez-protein","attrs":{"text":"AAB70931","term_id":"2429367","term_text":"AAB70931"}} AAB70931 ) R&D Systems, goat polyclonal, Cat# AF2645, RRID:AB_2110295 1:300 Neurofilament 200 (NF200) Neurofilaments of molecular weight 200 kDa in rat spinal cord extract Sigma-Aldrich, mouse monoclonal, Cat# N0142, RRID:AB_477257 1:4000 Neuropeptide Y (NPY) BTg-conjugated synthetic porcine NPY DiaSorin (now ImmunoStar), rabbit polyclonal, Cat# 22940, RRID:AB_572253 1:3000 Neuronal nitric oxide synthase (nNOS) Recombinant protein corresponding to 195 amino acids from the N-terminus of rat nNos Zymed (now Invitrogen), rabbit polyclonal, Cat# 61-7000, RRID:AB_2313734 1:500 S100 Purified bovine S100 Millipore, mouse monoclonal, Cat# MAB079-1, RRID:AB_571112 1:500 Smooth muscle actin-α (SMA) KLH-conjugated synthetic peptide corresponding to 10 amino acids from the N-terminus of SMA Sigma-Aldrich, mouse monoclonal, Cat# A5228 RRID:AB_262054 1:5000 Tyrosine hydroxylase (TH) Denatured tyrosine hydroxylase from rat pheaochromocytoma Millipore, rabbit polyclonal, Cat# AB152, RRID:AB_390204 1:1000 Vimentin (Vim) Porcine Vim purified from eye lens Sigma-Aldrich, mouse monoclonal, Cat# V6630, RRID:AB_477627 1:10,000 Open in a separate window Primary Antibodies Used for Immunohistochemistry Antibodies were diluted with 0.1M hypertonic PBS (pH 7.2).

Techniques: Isolation, Recombinant, Sequencing, Molecular Weight, Purification